Regulatory
Part:BBa_K3002027:Design
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-16)
pAR promoter for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a combination of the HSP70A and RBCS2-promoter, which leads to higher expressions compared to the RBCS2 promoter alone. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
The sequence was provided by the MoClo toolkit(pCM0-017-PAR_(Pro_A1-B2). ( Weber,E., 2011)