Regulatory

Part:BBa_K3002027:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)


pAR promoter for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a combination of the HSP70A and RBCS2-promoter, which leads to higher expressions compared to the RBCS2 promoter alone. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

The sequence was provided by the MoClo toolkit(pCM0-017-PAR_(Pro_A1-B2). ( Weber,E., 2011)


References